DARWIN Digitale Dissertationen German Version Strich

FU Berlin
Digitale Dissertation

Beatrix Barthel :
Functionell changes on red blood cells during storage in units of full blood or erythrocyte concentrates of the horse
Storage of horse blood units
Funktionelle Veränderungen der roten Blutzellen während ihrer Lagerung in Konserven mit Vollblut oder Erythrozytenkonzentraten vom Pferd

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Abstract

The short and long term storage of blood requires, among other things, optimal storage conditions for vital cells, in order to achieve a subsequent successful and effective transfusion.
Based on this fact we structured our investigations on horse blood claiming, that
(1) horse blood should verify the influence of various, differently composed preserving solutions as well as of different storing temperatures on the vital parameters of erythrocytes and, that (2) these results allow recommendations to optimize the storage of horse blood units.
Therefor blood was drawn from 16 healthy, adult horses. The preservation of this blood took place in form of combining either whole blood with a CPDA-1-Stabilizer, erythrocyte-concentrate-1 (ERY-1) with a SAG-M-Stabilizer or erythrozyte-concentrate-2 (ERY-2) with a GM-Solution (free of chloride ions). Precede we carryed out a depletion of chloride ions in ERY-2. The horse blood units were stored for either 4 days at 22°C or for 36 days at 4°C. We then determined following parameters in this blood units: hematology (RBC, Hb total, Hb extracellular, rate of hemolysis, Hct, MCV, MCHC, MCH, fragility of erythrocytes), hemoxymetry (pH, p50, O2Hb), electrolytes (sodium, potassium, chloride), enzyme (LDH), metabolites (glucose, lactate, 2,3-DPG, ATP). In total the whole blood and ERY-1 were still proper for a transfusion after storing periode of 4 days at temperature of 22°C as well as after storing time of 36 days at a temperature of 4°C. In ERY-2 the extracellular potassium level increased unjustifiably on day 4 at a temperature of 22°C (>50 mmol/l). So the use of this additive solution is not recommendable at 22°C. Until day 29 during the storing periode of 5 weeks at 4°C the ERY-2 was superior to the other Preserves, due to the fafourable progress of the erythrocytic vitality parameters.

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Online available: http://www.diss.fu-berlin.de/1999/77/indexe.html
Language of PhDThesis: german
Keywords: horse; horse blood; erythrocytes; storage; preservation; CPDA-1; CPD/SAG-M; deletion of chlorid; blood units
DNB-Sachgruppe: 34 Veterinärmedizin
Date of disputation: 17-Sep-1999
PhDThesis from: Fachbereich Veterinärmedizin, Freie Universität Berlin
First Referee: Univ.-Prof. Dr. H. Hartmann
Second Referee: Prof. Dr. G. Matthes
Date created:14-Dec-2000
Date available:21-Dec-2000

 

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