Abstract
The scientific paper on hand includes examinations to differing effects of Propolis in vitro and in vivo.
Seven per cent respectively 10 per cent dispensations ethanol Propolis-dryextract with glycerol respectively castor oils were successful put in once a day for local treatment by altogether 51 dogs with 97 otitis externa cases. There were 81,44 per cent of the cases healed after 7 days. The rate of cure reached 96,90 per cent after 14 days of treatment. There were no relapses identified within an observation period of 3 months.
Ethanol Propolis-dryextract effected dose-dependent antibacterial too bacterial on anaerobic bacterial strains. The tested germinal spectrum shows differences in the degree of sensitiveness of Propolis, conditioned by species. All 14 tested anaerobic bacterial strains completely stunted in growth by the dilution-test. The determined MIC-values of ethanol Propolis-dryextract for species of Fusobacterium were next to 1,0 mg Propolis-dryextract of each 1 ml culture, other tested anaerobic bacteria next to 0,5 mg per ml.
Propolis- and Poplarbudsdry-extract (from Populus italica) show pregnant effects on veterinary-medical relevant gram-positive bacteria, yeasts and funguses in the dilution-test. The antibacterial effect of Propolis- and Poplarbudsdry-extract was nearly the same on gram-positive germs. The antimycotic effect of Propolis-dryextract was a little higher, then by Poplarbuds-dryextract. The determined MIC-values of Propolis-dryextract and the Poplarbuds-dryextract by gram-positive bacteria, yeasts and mycosis had situated among 0,175 by Staphylococcus aureus, Trichophyton mentagrophytes and 2,8 mg per ml by Malassezia pachydermatis. The gram-negative germs Pseudomonas aeruginosa and Escherichia coli had not completely stunted in growth, testing concentrations till 2,8 mg per ml.
An antiviral effect depending on the concentration of Propolis-dryextract proved of Vesikular-stomatitis-virus from the family of Rhabdo-viruses and equine herpes virus 1 from the family of Herpesviridae. The strongest antiviral effect on both viruses had reached with a concentration of the the Propolis-dryextract of 125 µg per ml fluid culture medium. The antiviral effect of Propolis-dryextract on the EHV 1 was stronger marked, than on the VSV. There were quantitative determinations implemented by cELISA of the virus-proteins gp 51 and p 24. The results indicate a production-retardation of the bovine leucosis-virus by the addition of Propolis-dryextract to the fluid culture medium of the permanent cell culture FLK-BLV (VAN DER MAATEN et al. 1979).
All examined cell lines - the equine dermis cell line (ED), the bat lung cell line (bat), the fowl embryo fibroblaste cell line (HEF), the fetal lamb kidney cell line-BLV-infect (FLK/BLV) and the human immortalized cell line (HaCaT) - show cytotoxic effects, if the level of the Propolis-dryextract in the fluid culture medium was high.
The level of the Propolis-dryextract in the fluid culture medium, which lead to the complete cell-devastation depended on the cell line and amounted among 140 µg and 280 µg. The equine dermis cell line (ED) turned out most resistant to Propolis-dryextract. Small Propolis-dryextract-concentrations (below 10 µg per ml) lead by the cell lines FLK/BLV and HaCaT to a higher Proliferation. |
