FU Berlin Digitale Dissertation
Rüdiger Friedrich Wolf : Red blood cells drain with the CSF from the subarachnoid space into the cervical and lumbar paraaortic lymph nodes in rats Drainage von Erythrozyten aus dem Subarachnoidalraum in Lymphknoten - Eine tierexperimentelle Untersuchung zum Schädelhirntrauma
|Abstract| |Table of Contents| |More Information|

Abstract Both physiological and non-physiological studies have demonstrated the existence of CSF-drainage from the subarachnoid space into the deep cervical lymph nodes in the rat. In this study, we describe the distribution of red blood cells and their degradation by macrophages (erythrophages and siderophages) in lymph nodes in relation to the survival time after ?Controlled Cortical Impact Injury?(CCII). Lymph nodes were taken bilaterally from submandibular, deep cervical, lumbar paraaortic, inguinofemoral and popliteal sites in 38 Sprague-Dawley rats after CCII. Furthermore, the olfactory tract and the brainstem were dissected to examine the pathways of red blood cells within the subarachnoid space. Erythrophagocytosis and siderophagocytosis were visualized by hematoxylin & eosin, Goldner?s trichrome and Prussian blue reaction. Additionally, macrophages were immunohistochemically characterized by monoclonal ED1 antibody. To distinguish lymph node sinus from lymph node vessels, immunohistochemistry for cytoskeletal proteins was used. Red blood cells are visible in the marginal sinus of the cervical lymph nodes (consisting of superficial and deep nodes) as soon as 10 min after brain injury. After 16 h, the lumbar paraaortic lymph nodes showed the presence of red blood cells, too. Only one hour after appearance, erythrocytes had attached to macrophages in cervical and lumbar sites. Ingestion and digestion were seen immediately and until the third day post trauma. Then siderophages and iron pigment appeared in the medullar sinus. Adjacent to the olfactory tract as well as to the brainstem, erythrocytes were identified in the subarachnoid space. The time intervals concerning the presence of erythrocytes in the draining lymph nodes are identical to those discussed in other studies. This fact confirms the assumption that these red blood cells originate from traumatic subarachnoid hemorrhage at the brain injury site. The drainage of subarachnoid hemorrhage into cervical and lumbar paraaortic lymph nodes was demonstrated in this pathophysiological model of experimental trauma.

Table of Contents Download the whole PhDthesis as a zip-tar file or as zip-File For download in PDF format click the chapter title Inhaltsverzeichnis 4 1.  Einleitung 9 2.  Theoretischer Hintergrund 11 2.1.  Die Liquordrainage in das lymphatische System  11 2.2.  Der Lymphknoten 24 2.3.  Das Schädelhirntrauma 42 3.  Fragestellung 44 4.  Material und Methoden 46 4.1.  Das Traumamodell 46 4.2.  Herstellung von histologischen Schnitten 48 4.3.  Färbungen 50 4.4.  Immunhistochemie 52 4.5.  Elektronenmikroskopie 59 4.6.  Auswertung und Dokumentation 61 5.  Ergebnisse 62 5.1.  Makroskopische Untersuchung 62 5.2.  Mikroskopische Untersuchung 64 5.3.  Zusammenfassung der Ergebnisse 76 6.  Diskussion 80 6.1.  Die Liquordrainage in die tiefen cervikalen- und lumbalen paraaortalen Lymphknoten 81 6.2.  Erythrozyten-Makrophagen Interaktionen 92 6.3.  Methodendiskussion 95 6.4.  Ausblick 97 7.  Zusammenfassung  99 Literaturverzeichnis  101

More Information:
Online available:	http://www.diss.fu-berlin.de/2001/170/indexe.html
Language of PhDThesis:	german
Keywords:	cerebrospinal fluid; lymphatic drainage; traumatic brain injury; intracranial pressure; hemolymph nodes
DNB-Sachgruppe:	33 Medizin
Date of disputation:	12-Jun-2001
PhDThesis from:	Fachbereich Humanmedizin, Freie Universität Berlin
First Referee:	Prof. Dr.Gisela Stoltenburg-Didinger
Second Referee:	Priv.-Doz. Dr. M. Rothschild
Contact (Author):	ruedigerfwolf@gmx.de
Contact (Advisor):	gsd@mail.zedat.fu-berlin.de
Date created:	30-Aug-2001
Date available:	03-Sep-2001

 

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