FU Berlin Digitale Dissertation
Gerd Lindner : The role of hepatocyte growth factor/scatter factor in hair growth control Haarwachstumskontrolle durch den Hepatozyten-Wachstumsfaktor (HGF/SF)
|Abstract| |Table of Contents| |More Information|

Abstract Abstract:   HGF/SF and its receptor (Met) are principal mediators of mesenchymal- epithelial interactions in several different systems, and have recently been implicated in the control of hair follicle (HF) growth. Here, we have studied their expression patterns during HF morphogenesis and cycling in C57BL/6 mice, while functional hair growth effects of HGF/SF were assessed in vivo, by analysis of transgenic mice, and in skin organ culture. In normal mouse skin, follicular expression of HGF/SF and Met was strikingly localized: HGF/SF was found only in the HF mesenchyme (dermal papilla fibroblasts), Met in the neighboring hair bulb keratinocytes. Both HGF/SF and Met expression peaked during the initial phases of HF morphogenesis, the stage of active  hair growth (early and  mid anagen) and during the apoptosis-driven HF regression (catagen). Met+ cells in the regressing epithelial strand appeared to be protected from undergoing apoptosis (TUNEL+). Compared to wildtype controls, transgenic mice overexpressing HGF/SF under the control of the MT-1 promoter had twice as many developing HF and displayed accelerated HF development on postnatal day 3 (P3). They also showed a significant catagen retardation on P17. In both organ culture and in vivo, HGF/SF i.c. resulted in a significant catagen retardation. These results demonstrate an important role of HGF/SF and Met in murine hair growth and suggest that Met-mediated signaling  might be exploited for therapeutic manipulation of disorders of human hair.

Table of Contents Download the whole PhDthesis as a zip-tar file or as zip-File For download in PDF format click the chapter title Inhaltsübersicht 1. Einleitung.............................................................................................................. 2. Methoden............................................................................................................. 3. Materialien......................................................................................................... 4. Ergebnisse.......................................................................................................... 5. Diskussion......................................................................................................... 6. Literatur............................................................................................................. 7. Zusammenfassung........................................................................................... 8. Anhang................................................................................................................

More Information:
Online available:	http://www.diss.fu-berlin.de/2001/53/indexe.html
Language of PhDThesis:	german
Keywords:	HGF/SF, met, Haare, Haarwachstum, Haarausfall, Alopezie
DNB-Sachgruppe:	32 Biologie
Date of disputation:	27-Feb-2001
PhDThesis from:	Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin
First Referee:	Prof. Ralf Paus
Second Referee:	Prof. Ferdinand Hucho
Contact (Author):	glindner@uke.uni-hamburg.de
Contact (Advisor):	paus@uke.uni-hamburg.de
Date created:	20-Mar-2001
Date available:	06-Apr-2001

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