DARWIN Digitale Dissertationen German Version Strich

FU Berlin
Digitale Dissertation

Stephan Wehrenfennig :
NITRIC OXIDE SYNTHASE IN THE PERIPHERAL NERVOUS SYSTEM OF THE TURTLE TRACHEMYS SCRIPTA ELEGANS
STICKOXIDSYNTHASE IM PERIPHEREN NERVENSYSTEM DER SCHILDKRÖTE TRACHEMYS SCRIPTA ELEGANS

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Abstract

Nitric oxide has been identified as an important component of non-cholinergic, non-adrenergic neurotransmission in the peripheral nervous system of mammals. The present study is primarily concerned with the question to what extent the function of nitric oxide in autonomic transmission might represent a phylogenetically ancient and conserved feature. - Turtles were killed by an overdosis of pentobarbital and perfused transcadially by 4% paraformaldehyde. Cryostat sections were reacted for NADPH diaphorase histochemistry and with an antiserum against nitric oxide synthase (type I) followed by immunofluorescence or immunoperoxidase detection according to standard protocols. - While no positive cells were detected in a cervical sympathic ganglion, parasympathic ganglia (e.g. ciliary and palatine) and sensory ganglia (e.g. trigeminal) harbored different subpopulations of nitrergic neurons. In the respiratory tract, positive neurons were fund in the laryngeal and tracheal wall, in the nervus laryngeus recurrens and in intrapulmonary ganglia. The cardiac ganglia contained few reative neurons. Throughout the digestive tract, a considerable portion of neurons and fibers in the myenteric and submucosal plexus were stained. Many small positive ganglia were scatterred in the adrenal gland and around splenic blood vessels. Liver, kidney, pancreas and thymus showed no neuronal signals except of few paravasculary markings. With N-omega-nitro-L-arginin, a competitive antagonist of the nitric oxide synthase, preincubated sections showed no differnce in NADPH diaphorase histochemistry and immunofluorescence dection. In summary, the distribution pattern of neuronal nitric oxide synthase in the peripheral nervous system of a reptile reveals remarkable similarities to that of mammalian species. The use of nitric oxide as a transmitter appears to represent a phylogenetically ancient feature in automic regulation of organ function.

Table of Contents

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0. TITELBLATT, INHALT u. DANKSAGUNG
1. EINFÜHRUNG
1.1. Gliederung des peripheren Nervensystems 5
1.2. Neurotransmission im PNS 6
1.3. Synthese von Stickstoffmomoxid und Aufbau der Stickoxidsynthase 8
1.4. Enzymatischer Reaktionsablauf 11
1.5. Kolokalisation zu anderen Neurotransmittern 11
1.6. Spezifität der Antisera versus NADPH-Diaphorase-Aktivität 11
1.7. Steuerbarkeit und Funktion von NOS 12
1.8. Zielsetzung 13
2. MATERIAL UND METHODEN 14
2.1. Versuchstiere 14
2.2. Präparation 14
2.3. Anfertigung der Gewebeschnitte 15
2.4. Pufferlösungen 15
2.5. NADPH-Diaphorase-Histochemie 15
2.6. Indirekte Immunfluoreszenz 16
2.7. Peroxidase-Antiperoxidase 17
2.8. Kontrolluntersuchungen zu den immunologischen Versuchsreihen 18
2.9. Kontrolluntersuchungen zur Histochemie 18
2.10. Histologische Färbungen 19
2.11. Fotografische Dokumentation 21
2.12. Auswertung 22
3. ERGEBNISSE 23
3.1. Halsganglien 23
3.2. Sympathische Ganglien 26
3.3 Respiratorisches System 26
3.4. Verdauungstrakt 27
3.5. Ableitende Harnwege 31
3.6. Geschlechtsorgane 32
3.7. Drüsengewebe 33
3.8. Kardiovasculäres System 35
3.9. Lymphatische Organe 36
3.10. Nichtneuronale Markierungen 36
4. DISKUSSION 39
4.1. Methodik 39
4.2. Lokalisation von NO-Synthase im peripheren Nervensystem 43
5. ZUSAMMENFASSUNG 62
2. LITERATUR 64
7. ABKÜRZUNGEN 91
8. TABELLE UND ABBILDUNGEN 93
10. PUBLIKATION 114

More Information:

Online available: http://www.diss.fu-berlin.de/2002/161/indexe.html
Language of PhDThesis: german
Keywords: nitric oxide synthase, PNS, neurotransmission, turtle, NADPH diaphorase, NANC
DNB-Sachgruppe: 33 Medizin
Date of disputation: 11-Jun-2002
PhDThesis from: Fachbereich Humanmedizin, Freie Universität Berlin
First Referee: Privat Dozent Dr. Gerold Brüning
Second Referee: Prof. Dr. Ulrich Dirnagl
Contact (Author): wehrenfennig@web.de
Date created:05-Aug-2002
Date available:26-Aug-2002

 

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