FU Berlin Digitale Dissertation
Katinka Matz : Effects of an initial hemorrhagic shock on hemodynamic and clinical course of a subsequent Pseudomonas aeruginosa sepsis in a porcine "two-hit" model An experimental study with non-anaesthetized, chronic-invasive catheterized pigs Effekte eines initialen hämorrhagischen Schocks auf die Hämodynamik und den klinischen Verlauf einer nachfolgenden Pseudomonas aeruginosa Sepsis in einem porcinen "two-hit" model
|Abstract| |Table of Contents| |More Information|

Abstract A hemorrhagic shock is a potenially predisponent damage for a following bacterial sepsis. Therefore, the intention of this study was to establish a "two-hit" model with pigs characterized by a hemorrhagic shock as "first hit" and a Pseudomonas aeruginosa sepsis as "second hit". For this reason, twelve healthy juvenile pigs were first anaesthetized and catheterized by chronic-invasive means for hemodynamic takings and withdrawal of blood. Six animals were used as control group (n =6; no hemorrhagic shock). In 6 animals a hemorrhagic shock was induced by a 50% decrease of MAP and / or CI and maintained for 45 minutes. After volume replacement with cristalloid and colloidal volume expanders, retransfusion of the previously drawn blood was performed. 18 to 20 hours after end of narcosis, the chronically catheterized conscious pigs (n=12) were challenged with infusions of Pseudomonas aeruginosa (1,6 x 107 CFU/kg KGW/h first 24 hours followed by 1,6 x 106 CFU/kg KGW/h for the next 24 hours). Afterwards the pigs were supervised for 48 hours, then put down and dissected. In all animals bacterial sepsis could be induced. In comparison to the control group, the shock group showed the following significant differences in parameters investigated: · Higher scores in APACHE II classification immediately before sepsis induction. · Increased MPAP and PVRI during bacterial infusion. · Markedly elevated body temperature, decreased SVRI, increased MPAP and increased scores in APACHE II classification at the end of investigation time. Furthermore, lung and lymph nodes in lung of shock group animals were strongly populated by Pseudomonas aeruginosa (p = ns). It can be concluded from the "two-hit" model presented in this study, that the clinical course and hemodynamic effects of a Pseudomonas aeruginosa sepsis will be exacerbated by a preceding hemorrhagic shock.

Table of Contents Download the whole PhDthesis as a zip-tar file or as zip-File For download in PDF format click the chapter title Titelblatt, Inhaltsverzeichnis, Lebenslauf 1. Einleitung und Schrifttum 3. Material und Methoden 4. Ergebnisse 5. Diskussion 6. Zusammenfassung Literaturverzeichnis

More Information:
Online available:	http://www.diss.fu-berlin.de/2001/113/indexe.html
Language of PhDThesis:	german
Keywords:	Pigs; Animal Experiments; Shock; Septicaemia; Pseudomonas Aeruginosa
DNB-Sachgruppe:	34 Veterinärmedizin
Date of disputation:	27-Apr-2001
PhDThesis from:	Fachbereich Veterinärmedizin, Freie Universität Berlin
First Referee:	Univ.-Prof. Dr. L. Brunnberg
Second Referee:	PD Dr. J. Haberstroh
Third Referee:	Univ.-Prof. Dr. M.F.G. Schmidt
Contact (Advisor):	katinka.engel@netaddress.com
Date created:	20-Dec-2001
Date available:	17-Jan-2002

 

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