FU Berlin Digitale Dissertation
Holger Vogten : Central projection of calb d-28k immunopositive primary vestibular afferents in the mouse (mus musculus) Verlauf und zentrale Termination der Calbindin D-28k immunopositiven Subpopulation primärer vestibulärer Afferenzen in der Maus (Mus musculus)
|Abstract| |Table of Contents| |More Information|

Abstract The primary vestibular afferent neurons and fibers (PVA) contain a subgroup witch displays immunoreactivity for the calcium binding protein Calbindin D-28k (Calb). These Calb-positive PVA are constituted of especially large bipolar neurons in the vestibular ganglion and are characterized by large-diameter axons, an irregular spontaneous discharge patterns and predominantly phasic firing response to natural vestibular stimulation delivered through innervation of the central regions of the vestibular end organs via calyx-endings on type I vestibular hair cells. The present study describes the complete course and terminations of this subpopulation of Calb-positive PVA in the vestibular and cerebellar nuclei and the cerebellar cortex of the mouse. To prevent an overlapping in the staining by Calb-positive PVA from the contralateral side and by also Calb-positive cerebellar Purkinje cells (PC) and their axons, a unilateral transsection of the VIIIth nerve in the PC-deficient mutant mice Lurcher and Purkinje cell degeneration (pcd) was performed. One to three weeks after recovery they underwent intravital fixation. The fixed brains were cut in the coronal plane and subsequently processed for Calb immuncytochemistry. Vestibulectomy resulted in a complete loss of all ipsilateral Calb-positive fibers. The other side displayed the entire ipsilateral course of Calb-positive primary vestibular input including all terminations. All Calb-positive PVA and terminals are strictly unilateral. The fibers traverse all four vestibular nuclei except for the parvocellular part of the medial vestibular nucleus and they cross all three subdivisions of the cerebellar nuclei. Calb-positive terminations in the brainstem were restricted to the superior and to the inferior vestibular nucleus, to the ventral part of the lateral and to the lateral part of the medial vestibular nucleus, to the small cell group Y, to Cajal`s interstitial nucleus and to certain areas of the reticular formation. Calb-positive PVA terminations In the cerebellar nuclei were present in the medial and lateral cerebellar nuclei. Terminals in the cerebellar cortex were restricted only to the narrow posterior vermal areas of the rostral half of the ventral uvula and of the nodulus. Photographs of the preparates were taken, the results were evaluated, compared to those of other studies and discussed. Regarding the controversial discussion about the dimension of the vestibulocerebellum the present study can define the minimum distribution and extension of all PVA. In conclusion we found that the cerebellar mutant mice, Purkinje cell degeneration (pcd) and Lurcher, provide exellent tools to selectively investigate the complete subdivision of Calb-positive PVA including their terminals.

Table of Contents Download the whole PhDthesis as a zip-tar file or as zip-File For download in PDF format click the chapter title Titelseite und Inhaltsverzeichnis                Abkürzungen                        1. Einleitung 1.1 Vestibuläres System 1.2 Primär vestibuläre Afferenzen (PVA) 1.3 Vestibuläre Kerne (VN) 1.4 Cerebellum 1.5 Calbindin D-28k(Calb) im Gehirn 1.6 Problembeschreibung, Fragestellung und Zielsetzung       1.7 Auswahl der Versuchstiere   2. Materialien und Methoden   2.1 Versuchstiere 2.2 Operationen 2.3 Materialgewinnung      2.4 Schneiden 2.5 Immuncytochemie 2.6 Auswertung 3. Ergebnisse      3.1 Eigenschaften der Färbung mit anti-Calb und DAB       3.2 Verlauf von primär vestibulären Afferenzen im Hirnstamm       3.3 Termination von PVA im Hirnstamm 3.4 Faserverlauf und Termination von PVA im Kleinhirn       3.5 Termination von PVA im Kleinhirncortex 3.6 Weitere Calb-positive Zellen, Fasern und Artefakte       4. Diskussion 4.1 Methodenkritik 4.2 Methodenvergleich 4.3 Ergebnisvergleich 4.4 Bedeutung der Ergebnisse 5. Zusamenfassung und Summary           6. Literaturverzeichnis

More Information:
Online available:	http://www.diss.fu-berlin.de/2001/208/indexe.html
Language of PhDThesis:	german
Keywords:	vestibulocerebellum, primary vestibular afferents, vestibular projections, vestibular ganglion, type I haircells, vestibulotomy, calb d-28k, calcium binding proteins, pcd-mouse
DNB-Sachgruppe:	33 Medizin
Date of disputation:	09-Oct-2001
PhDThesis from:	Fachbereich Humanmedizin, Freie Universität Berlin
First Referee:	Prof. Dr. Ursula Grüsser-Cornehls
Second Referee:	Prof. Dr. Karl-Heinz Mauritz
Contact (Author):	holger.vogten@gmx.de
Date created:	30-Oct-2001
Date available:	30-Nov-2001

 

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