Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees
European Union DGXII Biotechnology FW IV Research Programme 
Molecular Tools for Biodiversity


Which DNA Marker for Which Purpose?

Final Compendium of the Research Project


Table of Contents

Objectives and concepts
  1.
The objectives of "Which DNA Marker for Which Purpose?"  -  E.M. Gillet, F. Scholz 
2.
DNA markers - Concepts and characteristics - E.M. Gillet 
3.
Purposes - Classification and desired marker characteristics - E.M. Gillet 

Inference of family relationships and mating system
  4.
Comparison of microsatellites and AFLP markers for parentage analysis  -  S. Gerber, S. Mariette, R. Streiff, C. Bodénès, A. Kremer 
5.
DIG-labelled AFLPs in oaks - A DNA marker for reconstruction of full- or half-sib family relationships?  -  B. Ziegenhagen, V. Kuhlenkamp, R. Brettschneider, F. Scholz, B.R. Stephan, B. Degen 
6.
Microsatellite analysis of anonymous seedlot samples from oak: a promising approach to monitor the number of different seed parents and pollen donors  -  C. Lexer, B. Heinze, S. Gerber, H. Steinkellner, B. Ziegenhagen, A. Kremer, J. Glössl 
7.
Nuclear microsatellites as a tool in the genetic characterization of forest reproductive material. A case study in sessile oak (Quercus petraea Matt., Liebl.)  -  S. La Scala, R. Schubert, G. Müller-Starck, K. Liepe 

Distribution of within-species genetic variation over space and time
8.
Microsatellite markers as a tool for the detection of intra- and interpopulation genetic structure  -  I. Scotti, G. Paglia, F. Magni, M. Morgante
9.
Chloroplast microsatellites for analysis of the geographic distribution of diversity in conifer species  -  M. Anzidei, A. Madaghiele, C. Sperisen, B. Ziegenhagen, G.G. Vendramin 
10. Mitochondrial DNA variation provides a tool for identifying introduced provenances: A case study in Norway spruce  -  C. Sperisen, U. Büchler, G. Mátyás, L. Ackzell 

Phylogeny reconstruction
11.
Limitations to the phylogenetic use of ITS sequences in closely related species and populations - a case study in Quercus petraea (Matt.) Liebl.  -  G. Muir, C. Schlötterer
12.
Amplified Fragment Length Polymorphisms and Microsatellites: A phylogenetic perspective  -  J.P. Robinson, S.A. Harris

Identification of expressed genes
13.
Isolation and sequence analysis of oak and spruce cDNA clones  -  M. Berenyi, S. Fluch, K. Hohl, K. Burg, R. Schubert, R. Riegel, G. Müller-Starck
14.
Application of the AFLP® technique in marker assisted breeding  -  J. Peleman


Which DNA Marker for Which Purpose? Final Compendium of the Research Project Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees" in the European Union DGXII Biotechnology FW IV Research Programme Molecular Tools for Biodiversity.  Gillet, E.M. (ed.). 1999.   URL http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker/index.htm

© Institut für Forstgenetik und Forstpflanzenzüchtung, Universität Göttingen, 1999